Not knowing what player you have, enz660, it is hard to say what would be the most effective method of prolonging runtime.
The Gigabeat F and X models don't have frequency scaling, so disabling audio features won't save any battery.
The coldfire targets (iRiver H3xx / H1xx, iAudio X5/M5) are more optimized (as I understand it) than the PortaPlayer targets (iPods, iRiver H10), and you can probably get away with some audio effects before seeing runtime decrease.
The PortaPlayer targets boost easily, and so it doesn't take much in the way of effects to decrease runtime.
Archos - I can't speak for them.
Something which will make a dramatic difference on all targets, though, is hard drive usage.
If you select an album, spin the hard drive to fill the buffer, then five minutes later change your mind, select a new album or add songs next in your playlist, you have wasted most of that first hard drive spin and incurred considerable battery costs.
Skipping tracks will also waste buffer that was (relatively) expensive to fill.